Generation and characterization of organoids from pluripotent stem cells and patient biopsy sample (normal and tumor)
Demonstrated experience in aseptic stem cell culture techniques: Isolation, culture, characterization, cryopreservation, and differentiation of adult stem cells and PSCs.
Confocal microscopy, Flow cytometry, FACS, qPCR
Immunological techniques: Western blot, Immunohistochemistry, ELISA, Immunocytochemistry, Double immunodiffusion
Invitro angiogenesis and wound healing assays
Anti-cancer, anti-toxicity, and cell viability studies